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KMID : 1149220120200020001
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Korean Journal of Oriental Medical Prescription
2012 Volume.20 No. 2 p.1 ~ p.11
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Comparative Study on Chemical Composition and Biological Activities of Samchulkunbi-tang Decoction and Commercial Herbal Medicine
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Kim Ohn-Soon
Seo Chang-Seob
Kim Ye-Ji
Shin Hyeun-Kyoo
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Abstract
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Objectives : The purpose of this study was to investigate the differences of chemical composition or biological activities between decoction and commercial herbal medicines of Samchulkunbi-tang (Shenzhujianpi-tang, SKT).
Methods : The extracts of SKT from decoction (SKT1) and two different commercial extractive granules (SKT2 and SKT3) were prepared. The index components of SKTs were analyzed with HPLC. The antioxidant activities of SKTs were studied by measuring free radical scavenging activities on ABTS and DPPH. The anti-inflammatory effects were determined by measuring NO, PGE2 and IL-6 in LPS-stimulated RAW 264.7 cells.
Results : The contents of 7 components were 1.40-6.08 mg/g in SKT1, not detected-4.75 mg/g in SKT2, 0.03-1.46 mg/g in SKT3. The scavenging activities on ABTS and DPPH of herbal formulas were increased in dose-dependent manner (SKT1>SKT2>SKT3). SKT1 significantly inhibited PGE2 and IL-6 production and SKT3 slightly inhibited PGE2 production in LPS-stimulated RAW264.7 cells. SKT2 showed no inhibitory effects on production of inflammatory mediators such as PGE2 and IL-6.
Conclusions : These results demonstrate that the decoction of SKT has more strong anti-oxidant and anti-inflammatory effects than that of commercial herbal medicines consistent with the contents of index components.
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KEYWORD
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Samchulkunbi-tang, index components, anti-oxidant effect, anti-inflammatory effect
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